Studying the growth boundary and subsequent time to growth of pathogenic Escherichia coli serotypes by turbidity measurements.

TítuloStudying the growth boundary and subsequent time to growth of pathogenic Escherichia coli serotypes by turbidity measurements.
Publication TypeJournal Article
Year of Publication2010
AuthorsValero, A, Rodríguez, M, Carrasco, E, Pérez-Rodríguez, F, García-Gimeno, RM, Zurera, G
JournalFood Microbiol
Volume27
Issue6
Pagination819-28
Date Published2010 Sep
ISSN1095-9998
Palabras claveAdaptation, Physiological, Colony Count, Microbial, Escherichia coli, Escherichia coli O157, Food Contamination, Food Microbiology, Hydrogen-Ion Concentration, Kinetics, Logistic Models, Models, Biological, Serotyping, Species Specificity, Temperature, Water
Abstract

The presence of Escherichia coli in contaminated food products is commonly attributed to faecal contamination when they are improperly handled and/or when inactivation treatments fail. Adaptation of E. coli at low pH and a(w) levels can vary at different temperatures depending on the serotype, thus more detailed studies are needed. In this work, a screening to assess the growth of four pathogenic serotypes of E. coli (O55:H6; O59:H21; O158:H23 and O157:H7) was performed. Subsequently, boundary models were elaborated with the fastest serotype selected at different temperatures (8, 12 and 16 degrees C), and inoculum levels (2, 3 and 4log cfu/mL) as function of pH (7.00-5.00) and a(w) (0.999-0.960). Finally, the growth kinetics of E. coli was described in the conditions that allowed growth. Results obtained showed that the serotypes O157:H7 and O59:H21 did not grow at more stringent conditions (8 degrees C; pH 5.50), while the E. coli O158:H23 was the best adapted, resulting in faster growth. The logistic regression models presented a good adjustment to data observed since more than 96.7% of cases were correctly classified. The growth interface was shifted to more limited conditions as the inoculum size was higher. Detection times (t(d), h) and their variability were higher at low levels of the environmental factors studied. This work provides insight on the growth kinetics of E. coli at various environmental conditions.

DOI10.1016/j.fm.2010.04.016
Alternate JournalFood Microbiol.